The biosynthesis of chondroitin-sulfate protein complex.
نویسندگان
چکیده
منابع مشابه
Biosynthesis of Chondroitin Sulfate
A microsomal preparation from chick embryo cartilage has been shown to catalyze the incorporation of sulfate-36S from 3’-phosphoadenosine 5’-phosphosulfate-3% into microsomal glycosaminoglycan. This enzyme preparation is the same preparation that previously has been shown to catalyze the formation of chondroitin (nonsulfated) from sugar nucleotide precursors. The microsomal sulfate acceptor app...
متن کاملBiosynthesis of Chondroitin Sulfate
The role of phospholipids in the activity of UDP-D-galactose:D-xylose galactosyltransferase (galactosyltransferase I) from embryonic chick cartilage was investigated. Phospholipase C treatment of particulate galactosyltransferase I caused inactivation of this enzyme to the extent of 60 to 70% as well as hydrolysis of 75 to 80% of the membrane phospholipids. Addition of phospholipid restored act...
متن کاملStudies on biosynthesis of the linkage region of chondroitin sulfate-protein complex.
In the native state, chondroitin-4-sulfate is covalently bound to protein. Since the original studies of M\uir,' which implicated serine in this linkage, Rod6n et al. in this laboratory have demonstrated that the linkage region between protein and polysaccharide in heparin,' chondroitin-4-sulfate,3' 4 and dermatan sulfate5 has the following structure: GlcUA-Gal-Gal-Xyl-serine. Other studies6'7 ...
متن کاملBiosynthesis of chondroitin sulfate. Organization of sulfation.
The potential relationship of an intact membrane organization for the synthesis of chondroitin and chondroitin 4-sulfate was examined after modification of a mouse mast cell microsomal system with the nonionic detergent, Triton X-100. The results indicated that Triton X-100 had no effect on the rate of polymerization but had a slight effect on the size of glycosaminoglycan chains. An "all or no...
متن کاملThe Biosynthesis of Chondroitin Sulfate by a Cell-free Preparation.
The biosynthesis of the acid mucopolysaccharide, hyaluronic acid, has been demonstrated in cell-free preparations derived from Group A streptococci (1, 2) and embryonic rat skin (3). In keeping with their function as glycosyl donors in many systems, the uridine nucleotides, uridine 5’-diphosphate-N-acetyln-glucosamine and UDP-n-glucuronic acid, have been shown to be the requisite intermediates ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1965
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.54.3.912